Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue

Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for fr...

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Detalles Bibliográficos
Autores principales: Mendelev, Natalia, Zamojski, Michel, Amper, Mary Anne S., Cheng, Wan Sze, Pincas, Hanna, Nair, Venugopalan D., Zaslavsky, Elena, Sealfon, Stuart C., Ruf-Zamojski, Frederique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184808/
https://www.ncbi.nlm.nih.gov/pubmed/35693209
http://dx.doi.org/10.1016/j.xpro.2022.101446
Descripción
Sumario:Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).

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