Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue

Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for fr...

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Autores principales: Mendelev, Natalia, Zamojski, Michel, Amper, Mary Anne S., Cheng, Wan Sze, Pincas, Hanna, Nair, Venugopalan D., Zaslavsky, Elena, Sealfon, Stuart C., Ruf-Zamojski, Frederique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184808/
https://www.ncbi.nlm.nih.gov/pubmed/35693209
http://dx.doi.org/10.1016/j.xpro.2022.101446
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author Mendelev, Natalia
Zamojski, Michel
Amper, Mary Anne S.
Cheng, Wan Sze
Pincas, Hanna
Nair, Venugopalan D.
Zaslavsky, Elena
Sealfon, Stuart C.
Ruf-Zamojski, Frederique
author_facet Mendelev, Natalia
Zamojski, Michel
Amper, Mary Anne S.
Cheng, Wan Sze
Pincas, Hanna
Nair, Venugopalan D.
Zaslavsky, Elena
Sealfon, Stuart C.
Ruf-Zamojski, Frederique
author_sort Mendelev, Natalia
collection PubMed
description Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022).
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spelling pubmed-91848082022-06-11 Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue Mendelev, Natalia Zamojski, Michel Amper, Mary Anne S. Cheng, Wan Sze Pincas, Hanna Nair, Venugopalan D. Zaslavsky, Elena Sealfon, Stuart C. Ruf-Zamojski, Frederique STAR Protoc Protocol Concomitant profiling of transcriptome and chromatin accessibility in isolated nuclei can reveal gene regulatory control mechanisms in health and disease. We report a single nucleus multi-omics analysis protocol optimized for frozen archived postmortem human pituitaries that is also effective for frozen ovine and murine pituitaries and human skeletal muscle biopsies. Its main advantages are that (1) it is not limited to fresh tissue, (2) it avoids tissue dissociation-induced transcriptional changes, and (3) it includes a novel, automated quality control pipeline. For complete details on the use and execution of this protocol, please refer to Ruf-Zamojski et al. (2021) and Zhang et al. (2022). Elsevier 2022-06-07 /pmc/articles/PMC9184808/ /pubmed/35693209 http://dx.doi.org/10.1016/j.xpro.2022.101446 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Mendelev, Natalia
Zamojski, Michel
Amper, Mary Anne S.
Cheng, Wan Sze
Pincas, Hanna
Nair, Venugopalan D.
Zaslavsky, Elena
Sealfon, Stuart C.
Ruf-Zamojski, Frederique
Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title_full Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title_fullStr Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title_full_unstemmed Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title_short Multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
title_sort multi-omics profiling of single nuclei from frozen archived postmortem human pituitary tissue
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184808/
https://www.ncbi.nlm.nih.gov/pubmed/35693209
http://dx.doi.org/10.1016/j.xpro.2022.101446
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