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Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches

Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characteriza...

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Detalles Bibliográficos
Autores principales: Schiro, Lars Erik, Bauer, Ulrich Stefan, Sandvig, Axel, Sandvig, Ioanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184809/
https://www.ncbi.nlm.nih.gov/pubmed/35693206
http://dx.doi.org/10.1016/j.xpro.2022.101426