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Isolation and comparison of neural stem cells from the adult rat brain and spinal cord canonical neurogenic niches
Here, we present a unified protocol for the extraction, culture, and basic characterization of rat neural stem cells (NSCs) from all three canonical neurogenic niches in the brain and spinal cord. We describe tissue dissection and dissociation, cell culture, followed by EdU labeling and characteriza...
Autores principales: | Schiro, Lars Erik, Bauer, Ulrich Stefan, Sandvig, Axel, Sandvig, Ioanna |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9184809/ https://www.ncbi.nlm.nih.gov/pubmed/35693206 http://dx.doi.org/10.1016/j.xpro.2022.101426 |
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