In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by...

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Detalles Bibliográficos
Autores principales: Sakamoto, Masayuki, Ota, Keisuke, Kondo, Yayoi, Okamura, Michiko, Fujii, Hajime, Bito, Haruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185017/
https://www.ncbi.nlm.nih.gov/pubmed/35693213
http://dx.doi.org/10.1016/j.xpro.2022.101421

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185017/
https://www.ncbi.nlm.nih.gov/pubmed/35693213
http://dx.doi.org/10.1016/j.xpro.2022.101421

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