In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice

We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by...

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Autores principales: Sakamoto, Masayuki, Ota, Keisuke, Kondo, Yayoi, Okamura, Michiko, Fujii, Hajime, Bito, Haruhiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185017/
https://www.ncbi.nlm.nih.gov/pubmed/35693213
http://dx.doi.org/10.1016/j.xpro.2022.101421
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author Sakamoto, Masayuki
Ota, Keisuke
Kondo, Yayoi
Okamura, Michiko
Fujii, Hajime
Bito, Haruhiko
author_facet Sakamoto, Masayuki
Ota, Keisuke
Kondo, Yayoi
Okamura, Michiko
Fujii, Hajime
Bito, Haruhiko
author_sort Sakamoto, Masayuki
collection PubMed
description We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022).
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spelling pubmed-91850172022-06-11 In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice Sakamoto, Masayuki Ota, Keisuke Kondo, Yayoi Okamura, Michiko Fujii, Hajime Bito, Haruhiko STAR Protoc Protocol We present a protocol to prepare mouse cranial window implantation for in vivo two-photon wide-field calcium imaging. This protocol uses G-CaMP9a transgenic mice, which express a genetically encoded calcium indicator with high signal-to-noise ratio. We describe in utero electroporation, followed by headplate fixation and cranial window implantation. This protocol can be used for measuring neural activity and is suitable for long-term imaging in large populations. Moreover, this protocol does not require preparation of Flp-expressing transgenic mice. For complete details on the use and execution of this protocol, please refer to Sakamoto et al. (2022). Elsevier 2022-06-07 /pmc/articles/PMC9185017/ /pubmed/35693213 http://dx.doi.org/10.1016/j.xpro.2022.101421 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sakamoto, Masayuki
Ota, Keisuke
Kondo, Yayoi
Okamura, Michiko
Fujii, Hajime
Bito, Haruhiko
In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title_full In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title_fullStr In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title_full_unstemmed In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title_short In utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using G-CaMP9a transgenic mice
title_sort in utero electroporation and cranial window implantation for in vivo wide-field two-photon calcium imaging using g-camp9a transgenic mice
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9185017/
https://www.ncbi.nlm.nih.gov/pubmed/35693213
http://dx.doi.org/10.1016/j.xpro.2022.101421
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