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Storage media and RNA extraction approaches substantially influence the recovery and integrity of livestock fecal microbial RNA

BACKGROUND: There is growing interest in understanding gut microbiome dynamics, to increase the sustainability of livestock production systems and to better understand the dynamics that regulate antibiotic resistance genes (i.e., the resistome). High-throughput sequencing of RNA transcripts (RNA-seq...

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Detalles Bibliográficos
Autores principales: Koorakula, Raju, Ghanbari, Mahdi, Schiavinato, Matteo, Wegl, Gertrude, Dohm, Juliane C., Domig, Konrad J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9186325/
https://www.ncbi.nlm.nih.gov/pubmed/35694379
http://dx.doi.org/10.7717/peerj.13547