HideRNAs protect against CRISPR-Cas9 re-cutting after successful single base-pair gene editing

Promiscuous activity of the Streptococcus pyogenes DNA nuclease CRISPR-Cas9 can result in destruction of a successfully modified sequence obtained by templated repair of a Cas9-induced DNA double-strand break. To avoid re-cutting, additional target-site-disruptions (TSDs) are often introduced on top...

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Detalles Bibliográficos
Autores principales: Harmsen, Tim J. W., Pritchard, Colin E. J., Riepsaame, Joey, van de Vrugt, Henri J., Huijbers, Ivo J., te Riele, Hein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187658/
https://www.ncbi.nlm.nih.gov/pubmed/35688932
http://dx.doi.org/10.1038/s41598-022-13688-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9187658/
https://www.ncbi.nlm.nih.gov/pubmed/35688932
http://dx.doi.org/10.1038/s41598-022-13688-y

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