Procedure of the overexpression, purification and crystallization of BLEG-1, a bifunctional and evolutionary divergent B3 metallo-β-lactamase, for structure-function studies

Metallo-β-lactamases (MBLs) are class B β-lactamases from the metallo-hydrolase-like MBL-fold superfamily which act on a broad range of β-lactam antibiotics, thus conferring antibiotics resistance to bacterial pathogens. The attempt to structurally characterize BLEG-1, an evolutionary divergent B3 m...

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Detalles Bibliográficos
Autores principales: Au, Shaw Xian, Noor, Noor Dina Muhd, Matsumura, Hiroyoshi, Rahman, Raja Noor Zaliha Raja Abdul, Normi, Yahaya M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Method Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189199/
https://www.ncbi.nlm.nih.gov/pubmed/35707637
http://dx.doi.org/10.1016/j.mex.2022.101740

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189199/
https://www.ncbi.nlm.nih.gov/pubmed/35707637
http://dx.doi.org/10.1016/j.mex.2022.101740

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