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Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence

Recent studies have shown that not only resistance, but also tolerance/persistence levels can evolve rapidly in bacteria exposed to repeated antibiotic treatments. We used in vitro evolution to assess whether tolerant/hyperpersistent Escherichia coli ATCC25922 mutants could be selected under repeate...

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Autores principales: Perault, Aurore, Turlan, Catherine, Eynard, Nathalie, Vallé, Quentin, Bousquet-Mélou, Alain, Giraud, Etienne
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189390/
https://www.ncbi.nlm.nih.gov/pubmed/35707170
http://dx.doi.org/10.3389/fmicb.2022.908296
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author Perault, Aurore
Turlan, Catherine
Eynard, Nathalie
Vallé, Quentin
Bousquet-Mélou, Alain
Giraud, Etienne
author_facet Perault, Aurore
Turlan, Catherine
Eynard, Nathalie
Vallé, Quentin
Bousquet-Mélou, Alain
Giraud, Etienne
author_sort Perault, Aurore
collection PubMed
description Recent studies have shown that not only resistance, but also tolerance/persistence levels can evolve rapidly in bacteria exposed to repeated antibiotic treatments. We used in vitro evolution to assess whether tolerant/hyperpersistent Escherichia coli ATCC25922 mutants could be selected under repeated exposure to a high ciprofloxacin concentration. Among two out of three independent evolution lines, we observed the emergence of gyrB mutants showing an hyperpersistence phenotype specific to fluoroquinolones, but no significant MIC increase. The identified mutation gives rise to a L422P substitution in GyrB, that is, outside of the canonical GyrB QRDR. Our results indicate that mutations in overlooked regions of quinolone target genes may impair the efficacy of treatments via an increase of persistence rather than resistance level, and support the idea that, in addition to resistance, phenotypes of tolerance/persistence of infectious bacterial strains should receive considerations in the choice of antibiotic therapies.
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spelling pubmed-91893902022-06-14 Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence Perault, Aurore Turlan, Catherine Eynard, Nathalie Vallé, Quentin Bousquet-Mélou, Alain Giraud, Etienne Front Microbiol Microbiology Recent studies have shown that not only resistance, but also tolerance/persistence levels can evolve rapidly in bacteria exposed to repeated antibiotic treatments. We used in vitro evolution to assess whether tolerant/hyperpersistent Escherichia coli ATCC25922 mutants could be selected under repeated exposure to a high ciprofloxacin concentration. Among two out of three independent evolution lines, we observed the emergence of gyrB mutants showing an hyperpersistence phenotype specific to fluoroquinolones, but no significant MIC increase. The identified mutation gives rise to a L422P substitution in GyrB, that is, outside of the canonical GyrB QRDR. Our results indicate that mutations in overlooked regions of quinolone target genes may impair the efficacy of treatments via an increase of persistence rather than resistance level, and support the idea that, in addition to resistance, phenotypes of tolerance/persistence of infectious bacterial strains should receive considerations in the choice of antibiotic therapies. Frontiers Media S.A. 2022-05-30 /pmc/articles/PMC9189390/ /pubmed/35707170 http://dx.doi.org/10.3389/fmicb.2022.908296 Text en Copyright © 2022 Perault, Turlan, Eynard, Vallé, Bousquet-Melou and Giraud. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Perault, Aurore
Turlan, Catherine
Eynard, Nathalie
Vallé, Quentin
Bousquet-Mélou, Alain
Giraud, Etienne
Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title_full Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title_fullStr Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title_full_unstemmed Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title_short Repeated Exposure of Escherichia coli to High Ciprofloxacin Concentrations Selects gyrB Mutants That Show Fluoroquinolone-Specific Hyperpersistence
title_sort repeated exposure of escherichia coli to high ciprofloxacin concentrations selects gyrb mutants that show fluoroquinolone-specific hyperpersistence
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189390/
https://www.ncbi.nlm.nih.gov/pubmed/35707170
http://dx.doi.org/10.3389/fmicb.2022.908296
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