A protocol to construct RNA-protein devices for photochemical translational regulation of synthetic mRNAs in mammalian cells

Here, we describe a protocol for the translational regulation of transfected messenger RNAs (mRNAs) using light in mammalian cells. We detail the steps for photocaged ligand synthesis, template DNA preparation, and mRNA synthesis. We describe steps for mRNA transfection, treatment of cells with a ph...

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Detalles Bibliográficos
Autores principales: Nakanishi, Hideyuki, Yoshii, Tatsuyuki, Tsukiji, Shinya, Saito, Hirohide
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189627/
https://www.ncbi.nlm.nih.gov/pubmed/35707682
http://dx.doi.org/10.1016/j.xpro.2022.101451

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189627/
https://www.ncbi.nlm.nih.gov/pubmed/35707682
http://dx.doi.org/10.1016/j.xpro.2022.101451

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