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Dual antibody strategy for high-resolution imaging of murine Purkinje cells and their dendrites across multiple layers

Despite being among the largest neurons in the mammalian brain, Purkinje cells are difficult to visualize and trace via immunofluorescence because their dendritic arbors extend through several cerebellar layers. This protocol describes a two-antibody strategy we developed to study Purkinje cell morp...

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Detalles Bibliográficos
Autores principales: Botta, Salvatore, Chemiakine, Alexei, Gennarino, Vincenzo A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189630/
https://www.ncbi.nlm.nih.gov/pubmed/35707684
http://dx.doi.org/10.1016/j.xpro.2022.101427