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Dual antibody strategy for high-resolution imaging of murine Purkinje cells and their dendrites across multiple layers
Despite being among the largest neurons in the mammalian brain, Purkinje cells are difficult to visualize and trace via immunofluorescence because their dendritic arbors extend through several cerebellar layers. This protocol describes a two-antibody strategy we developed to study Purkinje cell morp...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9189630/ https://www.ncbi.nlm.nih.gov/pubmed/35707684 http://dx.doi.org/10.1016/j.xpro.2022.101427 |