Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expa...

Descripción completa

Detalles Bibliográficos
Autores principales: Konishi, Satoshi, Tata, Aleksandra, Tata, Purushothama Rao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192963/
https://www.ncbi.nlm.nih.gov/pubmed/35712012
http://dx.doi.org/10.1016/j.xpro.2022.101447

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192963/
https://www.ncbi.nlm.nih.gov/pubmed/35712012
http://dx.doi.org/10.1016/j.xpro.2022.101447

Ejemplares similares