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Defined conditions for long-term expansion of murine and human alveolar epithelial stem cells in three-dimensional cultures

Alveolar type 2 cells (AT2s) serve as stem cells of the alveoli and restore cell numbers after injury. Here, we describe a detailed protocol for the isolation, purification, and culture of murine and human AT2s. We have developed chemically defined and stroma-free culture conditions that enable expa...

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Detalles Bibliográficos
Autores principales:	Konishi, Satoshi, Tata, Aleksandra, Tata, Purushothama Rao
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Elsevier 2022
Materias:	Protocol
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9192963/ https://www.ncbi.nlm.nih.gov/pubmed/35712012 http://dx.doi.org/10.1016/j.xpro.2022.101447

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