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Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismut...

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Detalles Bibliográficos
Autores principales: Du, Yuying, May, Robin C., Cooper, Helen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196203/
https://www.ncbi.nlm.nih.gov/pubmed/35617055
http://dx.doi.org/10.1039/d2cc02085h