Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies

Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismut...

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Detalles Bibliográficos
Autores principales: Du, Yuying, May, Robin C., Cooper, Helen J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196203/
https://www.ncbi.nlm.nih.gov/pubmed/35617055
http://dx.doi.org/10.1039/d2cc02085h
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author Du, Yuying
May, Robin C.
Cooper, Helen J.
author_facet Du, Yuying
May, Robin C.
Cooper, Helen J.
author_sort Du, Yuying
collection PubMed
description Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins.
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spelling pubmed-91962032022-06-27 Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies Du, Yuying May, Robin C. Cooper, Helen J. Chem Commun (Camb) Chemistry Here, we demonstrate that by combining electroporation with native ambient mass spectrometry, it is possible to detect intact non-covalent protein complexes directly from bacterial colonies growing on agar. Homodimers HdeA and HdeB were identified, together with the 50 kDa Mn-bound superoxide dismutase homodimer, in addition to some previously undetected monomeric proteins. The Royal Society of Chemistry 2022-05-24 /pmc/articles/PMC9196203/ /pubmed/35617055 http://dx.doi.org/10.1039/d2cc02085h Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Du, Yuying
May, Robin C.
Cooper, Helen J.
Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title_full Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title_fullStr Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title_full_unstemmed Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title_short Native ambient mass spectrometry of intact protein assemblies directly from Escherichia coli colonies
title_sort native ambient mass spectrometry of intact protein assemblies directly from escherichia coli colonies
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9196203/
https://www.ncbi.nlm.nih.gov/pubmed/35617055
http://dx.doi.org/10.1039/d2cc02085h
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AT cooperhelenj nativeambientmassspectrometryofintactproteinassembliesdirectlyfromescherichiacolicolonies

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