PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency

VP64 is the smallest transactivation domain that can be packaged together with the sgRNA into a single adeno-associated virus (AAV) vector. However, VP64-based CRISPRa often exerts modest activation to the target gene when only one sgRNA is used. Herein, we used PAM-flexible dual base editor-mediate...

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Detalles Bibliográficos
Autores principales: Lau, Cia-Hin, Huang, Siping, Lam, Raymond H.W., Tin, Chung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198377/
https://www.ncbi.nlm.nih.gov/pubmed/35755943
http://dx.doi.org/10.1016/j.omtm.2022.05.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198377/
https://www.ncbi.nlm.nih.gov/pubmed/35755943
http://dx.doi.org/10.1016/j.omtm.2022.05.005

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