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PAM-flexible dual base editor-mediated random mutagenesis and self-activation strategies to improve CRISPRa potency
VP64 is the smallest transactivation domain that can be packaged together with the sgRNA into a single adeno-associated virus (AAV) vector. However, VP64-based CRISPRa often exerts modest activation to the target gene when only one sgRNA is used. Herein, we used PAM-flexible dual base editor-mediate...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Gene & Cell Therapy
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9198377/ https://www.ncbi.nlm.nih.gov/pubmed/35755943 http://dx.doi.org/10.1016/j.omtm.2022.05.005 |