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Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique

Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation. We describe the procedures of in utero electroporation and brain slide preparation. We then detail the procedures o...

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Detalles Bibliográficos
Autores principales: Chen, Liping, Li, Ying, Wu, Yan, Li, Shen, Chang, Xin, Wu, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201042/
https://www.ncbi.nlm.nih.gov/pubmed/35719723
http://dx.doi.org/10.1016/j.xpro.2022.101461