Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique

Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation. We describe the procedures of in utero electroporation and brain slide preparation. We then detail the procedures o...

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Detalles Bibliográficos
Autores principales: Chen, Liping, Li, Ying, Wu, Yan, Li, Shen, Chang, Xin, Wu, Haitao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201042/
https://www.ncbi.nlm.nih.gov/pubmed/35719723
http://dx.doi.org/10.1016/j.xpro.2022.101461
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author Chen, Liping
Li, Ying
Wu, Yan
Li, Shen
Chang, Xin
Wu, Haitao
author_facet Chen, Liping
Li, Ying
Wu, Yan
Li, Shen
Chang, Xin
Wu, Haitao
author_sort Chen, Liping
collection PubMed
description Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation. We describe the procedures of in utero electroporation and brain slide preparation. We then detail the procedures of senescence-associated β-galactosidase (SA-β-Gal) staining and immunofluorescence staining, followed by microscope imaging analysis. This combined approach can be used to study the in situ senescence of in utero electroporated embryonic brains. For complete details on the use and execution of this protocol, please refer to Zhu et al. (2021).
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spelling pubmed-92010422022-06-17 Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique Chen, Liping Li, Ying Wu, Yan Li, Shen Chang, Xin Wu, Haitao STAR Protoc Protocol Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation. We describe the procedures of in utero electroporation and brain slide preparation. We then detail the procedures of senescence-associated β-galactosidase (SA-β-Gal) staining and immunofluorescence staining, followed by microscope imaging analysis. This combined approach can be used to study the in situ senescence of in utero electroporated embryonic brains. For complete details on the use and execution of this protocol, please refer to Zhu et al. (2021). Elsevier 2022-06-13 /pmc/articles/PMC9201042/ /pubmed/35719723 http://dx.doi.org/10.1016/j.xpro.2022.101461 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Chen, Liping
Li, Ying
Wu, Yan
Li, Shen
Chang, Xin
Wu, Haitao
Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title_full Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title_fullStr Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title_full_unstemmed Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title_short Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
title_sort protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201042/
https://www.ncbi.nlm.nih.gov/pubmed/35719723
http://dx.doi.org/10.1016/j.xpro.2022.101461
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