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Protocol for analysis of senescent neuronal stem cells in genetic-modified embryonic mice using in utero electroporation technique
Here, we present a detailed protocol for identification and analysis of senescent neuronal stem cells (NSCs) in the developing mouse embryonic neocortex using in utero electroporation. We describe the procedures of in utero electroporation and brain slide preparation. We then detail the procedures o...
Autores principales: | Chen, Liping, Li, Ying, Wu, Yan, Li, Shen, Chang, Xin, Wu, Haitao |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201042/ https://www.ncbi.nlm.nih.gov/pubmed/35719723 http://dx.doi.org/10.1016/j.xpro.2022.101461 |
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