Tandem immuno-purification of affinity-tagged proteins from mouse testis extracts for MS analysis

Here, we describe a protocol for extraction and tandem immunoprecipitation of the cytoplasmic and nuclear proteins from mouse testis for mass spectrometry. This protocol has been applied to knockin mice that express a meiotic protein of interest tagged with 3xFLAG-HA in the testis. The protocol is o...

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Detalles Bibliográficos
Autores principales: Tani, Naoki, Tanno, Nobuhiro, Ishiguro, Kei-ichiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201064/
https://www.ncbi.nlm.nih.gov/pubmed/35719267
http://dx.doi.org/10.1016/j.xpro.2022.101452

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201064/
https://www.ncbi.nlm.nih.gov/pubmed/35719267
http://dx.doi.org/10.1016/j.xpro.2022.101452

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