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One-pot platform for rapid detecting virus utilizing recombinase polymerase amplification and CRISPR/Cas12a

ABSTRACT: The livestock industry has been deeply affected by African swine fever virus (ASFV) and Capripoxvirus (CaPV), which caused an enormous economic damage. It is emergent to develop a reliable detection method. Here, we developed a rapid, ultra-sensitive, and one-pot DNA detection method combi...

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Detalles Bibliográficos
Autores principales: Xiong, Yifan, Cao, Gaihua, Chen, Xiaolong, Yang, Jun, Shi, Meimei, Wang, Yu, Nie, Fuping, Huo, Danqun, Hou, Changjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9201268/
https://www.ncbi.nlm.nih.gov/pubmed/35708748
http://dx.doi.org/10.1007/s00253-022-12015-9