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Experimental Characterization of In Silico Red-Shift-Predicted iLOV(L470T/Q489K) and iLOV(V392K/F410V/A426S) Mutants

[Image: see text] iLOV is a flavin mononucleotide-binding fluorescent protein used for in vivo cellular imaging similar to the green fluorescent protein. To expand the range of applications of iLOV, spectrally tuned red-shifted variants are desirable to reduce phototoxicity and allow for better tiss...

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Detalles Bibliográficos
Autores principales: Wehler, Pierre, Armbruster, Daniel, Günter, Andreas, Schleicher, Erik, Di Ventura, Barbara, Öztürk, Mehmet Ali
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202016/
https://www.ncbi.nlm.nih.gov/pubmed/35722011
http://dx.doi.org/10.1021/acsomega.2c01283