An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization

The sea urchin egg cortex is a peripheral region of eggs comprising a cell membrane and adjacent cytoplasm, which contains actin and tubulin cytoskeleton, cortical granules and some proteins required for early development. Method for isolation of cortices from sea urchin eggs and early embryos was d...

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Detalles Bibliográficos
Autores principales: Kipryushina, Yulia O., Maiorova, Mariia A., Yakovlev, Konstantin V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202947/
https://www.ncbi.nlm.nih.gov/pubmed/35709154
http://dx.doi.org/10.1371/journal.pone.0260831

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202947/
https://www.ncbi.nlm.nih.gov/pubmed/35709154
http://dx.doi.org/10.1371/journal.pone.0260831

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