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An approach to quantitate maternal transcripts localized in sea urchin egg cortex using RT-qPCR with accurate normalization
The sea urchin egg cortex is a peripheral region of eggs comprising a cell membrane and adjacent cytoplasm, which contains actin and tubulin cytoskeleton, cortical granules and some proteins required for early development. Method for isolation of cortices from sea urchin eggs and early embryos was d...
| Autores principales: | , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9202947/ https://www.ncbi.nlm.nih.gov/pubmed/35709154 http://dx.doi.org/10.1371/journal.pone.0260831 |