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Protocol for engineering and validating a synthetic mitochondrial intermembrane bridge in mammalian cells

Membrane contact sites are recognized as critical means of intercompartmental communication. Here, we describe a protocol for engineering and validating a synthetic bridge between the inner and outer mitochondrial membranes to support functioning of the endogenous mitochondrial contact site and cris...

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Detalles Bibliográficos
Autores principales: Purohit, Gunjan, Viana, Martonio Ponte, Khalimonchuk, Oleh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204730/
https://www.ncbi.nlm.nih.gov/pubmed/35719722
http://dx.doi.org/10.1016/j.xpro.2022.101454