Activity-based protein profiling of a surfactin-producing nonribosomal peptide synthetase in Bacillus subtilis

We present an in vitro and in-cell activity-based protein profiling (ABPP) protocol for endogenous nonribosomal peptide synthetases (NRPSs). This protocol enables the fluorescence labeling and imaging of an endogenous SrfAB-NRPS with high selectivity and sensitivity in the surfactin producer Bacillu...

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Detalles Bibliográficos
Autores principales: Ishikawa, Fumihiro, Ohnishi, Rina, Uchida, Chiharu, Tanabe, Genzoh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204745/
https://www.ncbi.nlm.nih.gov/pubmed/35719724
http://dx.doi.org/10.1016/j.xpro.2022.101462

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204745/
https://www.ncbi.nlm.nih.gov/pubmed/35719724
http://dx.doi.org/10.1016/j.xpro.2022.101462

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