Protocol for construction and characterization of direct electron transfer-based enzyme-electrode using gold binding peptide as molecular binder

Here, we present a protocol for constructing direct electron transfer (DET)-based enzyme-electrodes using gold-binding peptide (GBP). We describe fusion of four GBPs to flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase gamma-alpha complex (GDHγα), as model oxidoreductase, to generate...

Descripción completa

Detalles Bibliográficos
Autores principales: Lee, Hyeryeong, Lee, Eun Mi, Reginald, Stacy Simai, Chang, In Seop
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204793/
https://www.ncbi.nlm.nih.gov/pubmed/35719727
http://dx.doi.org/10.1016/j.xpro.2022.101466

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204793/
https://www.ncbi.nlm.nih.gov/pubmed/35719727
http://dx.doi.org/10.1016/j.xpro.2022.101466

Ejemplares similares