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Protocol for construction and characterization of direct electron transfer-based enzyme-electrode using gold binding peptide as molecular binder
Here, we present a protocol for constructing direct electron transfer (DET)-based enzyme-electrodes using gold-binding peptide (GBP). We describe fusion of four GBPs to flavin adenine dinucleotide (FAD)-dependent glucose dehydrogenase gamma-alpha complex (GDHγα), as model oxidoreductase, to generate...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204793/ https://www.ncbi.nlm.nih.gov/pubmed/35719727 http://dx.doi.org/10.1016/j.xpro.2022.101466 |