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A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells

Patients with human papillomavirus-positive squamous cell carcinoma of the head and neck (HPV+ HNSCC) have a favorable prognosis compared to those with HPV-negative (HPV−) ones. We have shown previously that HPV+ HNSCC cell lines are characterized by enhanced radiation sensitivity and impaired DNA d...

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Autores principales: Köcher, Sabrina, Zech, Henrike Barbara, Krug, Leonie, Gatzemeier, Fruzsina, Christiansen, Sabrina, Meyer, Felix, Rietow, Ruth, Struve, Nina, Mansour, Wael Yassin, Kriegs, Malte, Petersen, Cordula, Betz, Christian, Rothkamm, Kai, Rieckmann, Thorsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204973/
https://www.ncbi.nlm.nih.gov/pubmed/35719921
http://dx.doi.org/10.3389/fonc.2022.765968
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author Köcher, Sabrina
Zech, Henrike Barbara
Krug, Leonie
Gatzemeier, Fruzsina
Christiansen, Sabrina
Meyer, Felix
Rietow, Ruth
Struve, Nina
Mansour, Wael Yassin
Kriegs, Malte
Petersen, Cordula
Betz, Christian
Rothkamm, Kai
Rieckmann, Thorsten
author_facet Köcher, Sabrina
Zech, Henrike Barbara
Krug, Leonie
Gatzemeier, Fruzsina
Christiansen, Sabrina
Meyer, Felix
Rietow, Ruth
Struve, Nina
Mansour, Wael Yassin
Kriegs, Malte
Petersen, Cordula
Betz, Christian
Rothkamm, Kai
Rieckmann, Thorsten
author_sort Köcher, Sabrina
collection PubMed
description Patients with human papillomavirus-positive squamous cell carcinoma of the head and neck (HPV+ HNSCC) have a favorable prognosis compared to those with HPV-negative (HPV−) ones. We have shown previously that HPV+ HNSCC cell lines are characterized by enhanced radiation sensitivity and impaired DNA double-strand break (DSB) repair. Since then, various publications have suggested a defect in homologous recombination (HR) and dysregulated expression of DSB repair proteins as underlying mechanisms, but conclusions were often based on very few cell lines. When comparing the expression levels of suggested proteins and other key repair factors in 6 HPV+ vs. 5 HPV− HNSCC strains, we could not confirm most of the published differences. Furthermore, HPV+ HNSCC strains did not demonstrate enhanced sensitivity towards PARP inhibition, questioning a general HR defect. Interestingly, our expression screen revealed minimal levels of the central DNA damage response kinase ATM in the two most radiosensitive HPV+ strains. We therefore tested whether insufficient ATM activity may contribute to the enhanced cellular radiosensitivity. Irrespective of their ATM expression level, radiosensitive HPV+ HNSCC cells displayed DSB repair kinetics similar to ATM-deficient cells. Upon ATM inhibition, HPV+ cell lines showed only a marginal increase in residual radiation-induced γH2AX foci and induction of G2 cell cycle arrest as compared to HPV− ones. In line with these observations, ATM inhibition sensitized HPV+ HNSCC strains less towards radiation than HPV− strains, resulting in similar levels of sensitivity. Unexpectedly, assessment of the phosphorylation kinetics of the ATM targets KAP-1 and Chk2 as well as ATM autophosphorylation after radiation did not indicate directly compromised ATM activity in HPV-positive cells. Furthermore, ATM inhibition delayed radiation induced DNA end resection in both HPV+ and HPV− cells to a similar extent, further suggesting comparable functionality. In conclusion, DNA repair kinetics and a reduced effectiveness of ATM inhibition clearly point to an impaired ATM-orchestrated DNA damage response in HPV+ HNSCC cells, but since ATM itself is apparently functional, the molecular mechanisms need to be further explored.
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spelling pubmed-92049732022-06-18 A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells Köcher, Sabrina Zech, Henrike Barbara Krug, Leonie Gatzemeier, Fruzsina Christiansen, Sabrina Meyer, Felix Rietow, Ruth Struve, Nina Mansour, Wael Yassin Kriegs, Malte Petersen, Cordula Betz, Christian Rothkamm, Kai Rieckmann, Thorsten Front Oncol Oncology Patients with human papillomavirus-positive squamous cell carcinoma of the head and neck (HPV+ HNSCC) have a favorable prognosis compared to those with HPV-negative (HPV−) ones. We have shown previously that HPV+ HNSCC cell lines are characterized by enhanced radiation sensitivity and impaired DNA double-strand break (DSB) repair. Since then, various publications have suggested a defect in homologous recombination (HR) and dysregulated expression of DSB repair proteins as underlying mechanisms, but conclusions were often based on very few cell lines. When comparing the expression levels of suggested proteins and other key repair factors in 6 HPV+ vs. 5 HPV− HNSCC strains, we could not confirm most of the published differences. Furthermore, HPV+ HNSCC strains did not demonstrate enhanced sensitivity towards PARP inhibition, questioning a general HR defect. Interestingly, our expression screen revealed minimal levels of the central DNA damage response kinase ATM in the two most radiosensitive HPV+ strains. We therefore tested whether insufficient ATM activity may contribute to the enhanced cellular radiosensitivity. Irrespective of their ATM expression level, radiosensitive HPV+ HNSCC cells displayed DSB repair kinetics similar to ATM-deficient cells. Upon ATM inhibition, HPV+ cell lines showed only a marginal increase in residual radiation-induced γH2AX foci and induction of G2 cell cycle arrest as compared to HPV− ones. In line with these observations, ATM inhibition sensitized HPV+ HNSCC strains less towards radiation than HPV− strains, resulting in similar levels of sensitivity. Unexpectedly, assessment of the phosphorylation kinetics of the ATM targets KAP-1 and Chk2 as well as ATM autophosphorylation after radiation did not indicate directly compromised ATM activity in HPV-positive cells. Furthermore, ATM inhibition delayed radiation induced DNA end resection in both HPV+ and HPV− cells to a similar extent, further suggesting comparable functionality. In conclusion, DNA repair kinetics and a reduced effectiveness of ATM inhibition clearly point to an impaired ATM-orchestrated DNA damage response in HPV+ HNSCC cells, but since ATM itself is apparently functional, the molecular mechanisms need to be further explored. Frontiers Media S.A. 2022-05-31 /pmc/articles/PMC9204973/ /pubmed/35719921 http://dx.doi.org/10.3389/fonc.2022.765968 Text en Copyright © 2022 Köcher, Zech, Krug, Gatzemeier, Christiansen, Meyer, Rietow, Struve, Mansour, Kriegs, Petersen, Betz, Rothkamm and Rieckmann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Köcher, Sabrina
Zech, Henrike Barbara
Krug, Leonie
Gatzemeier, Fruzsina
Christiansen, Sabrina
Meyer, Felix
Rietow, Ruth
Struve, Nina
Mansour, Wael Yassin
Kriegs, Malte
Petersen, Cordula
Betz, Christian
Rothkamm, Kai
Rieckmann, Thorsten
A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title_full A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title_fullStr A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title_full_unstemmed A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title_short A Lack of Effectiveness in the ATM-Orchestrated DNA Damage Response Contributes to the DNA Repair Defect of HPV-Positive Head and Neck Cancer Cells
title_sort lack of effectiveness in the atm-orchestrated dna damage response contributes to the dna repair defect of hpv-positive head and neck cancer cells
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9204973/
https://www.ncbi.nlm.nih.gov/pubmed/35719921
http://dx.doi.org/10.3389/fonc.2022.765968
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