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Highly efficient libraries design for saturation mutagenesis

Saturation mutagenesis is a semi-rational approach for protein engineering where sites are saturated either entirely or partially to include amino acids of interest. We previously reported on a codon compression algorithm, where a set of minimal degenerate codons are selected according to user-defin...

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Detalles Bibliográficos
Autores principales: Pines, Gur, Pines, Assaf, Eckert, Carrie A
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9205323/
https://www.ncbi.nlm.nih.gov/pubmed/35734540
http://dx.doi.org/10.1093/synbio/ysac006