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The optimal dietary arginine level of laying hens fed with low-protein diets
BACKGROUND: Arginine (Arg) is an essential amino acid (EAA) in poultry, an important substrate for protein synthesis and a precursor of several molecules. Supplementation of EAAs with low protein (LP) diet increases the utilization efficiency of dietary crude protein (CP). However, if the EAA requir...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206374/ https://www.ncbi.nlm.nih.gov/pubmed/35715827 http://dx.doi.org/10.1186/s40104-022-00719-x |
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author | Sun, Mingfa Ma, Ning Liu, Hui Liu, Yu Zhou, Yunlei Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Ma, Baishun Jiao, Hongchao Lin, Hai |
author_facet | Sun, Mingfa Ma, Ning Liu, Hui Liu, Yu Zhou, Yunlei Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Ma, Baishun Jiao, Hongchao Lin, Hai |
author_sort | Sun, Mingfa |
collection | PubMed |
description | BACKGROUND: Arginine (Arg) is an essential amino acid (EAA) in poultry, an important substrate for protein synthesis and a precursor of several molecules. Supplementation of EAAs with low protein (LP) diet increases the utilization efficiency of dietary crude protein (CP). However, if the EAA requirement is changed in hens fed a LP diet remains to be elucidated. The aim of the present study was to evaluate the optimal level of dietary Arg in the LP diet of hens. A total of 1350 Hy-Line Brown laying hens were randomly allocated to six dietary treatments: a basal diet (16% CP, positive control), or an isoenergetic LP diet (14% CP, 0.80% Arg) supplemented 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg, corresponding to 0.80%, 0.85%, 0.90%, 0.95% and 1.00% dietary Arg, respectively. RESULTS: The feed efficiency was decreased (P < 0.05) by 0.80% and 1.00% Arg-LP diets, compared to control. Within LP diets, dietary Arg level had significant quadratic effects (P < 0.05) on laying rate, egg mass, and feed efficiency. Compared to control, the plasma CAT activity or T-AOC content were decreased by 0.80% (P < 0.001). However, the hens offered 0.85% and 0.90% Arg-LP diets had higher CAT activity (P < 0.001) than 0.80% Arg-LP diet. In contrast, 1.00% Arg-LP group had the highest MDA and the lowest T-AOC content in plasma, liver, duodenal and jejunal mucosa (P < 0.05). Compared to control, the villus height was decreased by 0.80%, 0.95% and 1.00% Arg-LP diets, while the villus height to crypt depth (V/C) ratio was reduced by 0.95% and 1.00% Arg-LP diets in duodenum. CONCLUSION: The result demonstrates that LP diet (14% CP) deficient in Arg (0.80% Arg) result in augmented oxidative damage and impaired development of intestinal mucosa. According to the quadratic broken-line regression model, the optimal dietary arginine levels for Hy-Line Brown laying hens fed with low protein diet (14% CP) aged 33 to 40 weeks are 0.85%, 0.86%, and 0.86% to obtained the maximum laying rate, egg mass, and feed efficiency, respectively. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-022-00719-x. |
format | Online Article Text |
id | pubmed-9206374 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-92063742022-06-19 The optimal dietary arginine level of laying hens fed with low-protein diets Sun, Mingfa Ma, Ning Liu, Hui Liu, Yu Zhou, Yunlei Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Ma, Baishun Jiao, Hongchao Lin, Hai J Anim Sci Biotechnol Research BACKGROUND: Arginine (Arg) is an essential amino acid (EAA) in poultry, an important substrate for protein synthesis and a precursor of several molecules. Supplementation of EAAs with low protein (LP) diet increases the utilization efficiency of dietary crude protein (CP). However, if the EAA requirement is changed in hens fed a LP diet remains to be elucidated. The aim of the present study was to evaluate the optimal level of dietary Arg in the LP diet of hens. A total of 1350 Hy-Line Brown laying hens were randomly allocated to six dietary treatments: a basal diet (16% CP, positive control), or an isoenergetic LP diet (14% CP, 0.80% Arg) supplemented 0, 0.05%, 0.10%, 0.15%, and 0.20% L-Arg, corresponding to 0.80%, 0.85%, 0.90%, 0.95% and 1.00% dietary Arg, respectively. RESULTS: The feed efficiency was decreased (P < 0.05) by 0.80% and 1.00% Arg-LP diets, compared to control. Within LP diets, dietary Arg level had significant quadratic effects (P < 0.05) on laying rate, egg mass, and feed efficiency. Compared to control, the plasma CAT activity or T-AOC content were decreased by 0.80% (P < 0.001). However, the hens offered 0.85% and 0.90% Arg-LP diets had higher CAT activity (P < 0.001) than 0.80% Arg-LP diet. In contrast, 1.00% Arg-LP group had the highest MDA and the lowest T-AOC content in plasma, liver, duodenal and jejunal mucosa (P < 0.05). Compared to control, the villus height was decreased by 0.80%, 0.95% and 1.00% Arg-LP diets, while the villus height to crypt depth (V/C) ratio was reduced by 0.95% and 1.00% Arg-LP diets in duodenum. CONCLUSION: The result demonstrates that LP diet (14% CP) deficient in Arg (0.80% Arg) result in augmented oxidative damage and impaired development of intestinal mucosa. According to the quadratic broken-line regression model, the optimal dietary arginine levels for Hy-Line Brown laying hens fed with low protein diet (14% CP) aged 33 to 40 weeks are 0.85%, 0.86%, and 0.86% to obtained the maximum laying rate, egg mass, and feed efficiency, respectively. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40104-022-00719-x. BioMed Central 2022-06-17 /pmc/articles/PMC9206374/ /pubmed/35715827 http://dx.doi.org/10.1186/s40104-022-00719-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Sun, Mingfa Ma, Ning Liu, Hui Liu, Yu Zhou, Yunlei Zhao, Jingpeng Wang, Xiaojuan Li, Haifang Ma, Baishun Jiao, Hongchao Lin, Hai The optimal dietary arginine level of laying hens fed with low-protein diets |
title | The optimal dietary arginine level of laying hens fed with low-protein diets |
title_full | The optimal dietary arginine level of laying hens fed with low-protein diets |
title_fullStr | The optimal dietary arginine level of laying hens fed with low-protein diets |
title_full_unstemmed | The optimal dietary arginine level of laying hens fed with low-protein diets |
title_short | The optimal dietary arginine level of laying hens fed with low-protein diets |
title_sort | optimal dietary arginine level of laying hens fed with low-protein diets |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206374/ https://www.ncbi.nlm.nih.gov/pubmed/35715827 http://dx.doi.org/10.1186/s40104-022-00719-x |
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