Peptide fusion improves prime editing efficiency

Prime editing enables search-and-replace genome editing but is limited by low editing efficiency. We present a high-throughput approach, the Peptide Self-Editing sequencing assay (PepSEq), to measure how fusion of 12,000 85-amino acid peptides influences prime editing efficiency. We show that peptid...

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Detalles Bibliográficos
Autores principales: Velimirovic, Minja, Zanetti, Larissa C., Shen, Max W., Fife, James D., Lin, Lin, Cha, Minsun, Akinci, Ersin, Barnum, Danielle, Yu, Tian, Sherwood, Richard I.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206660/
https://www.ncbi.nlm.nih.gov/pubmed/35717416
http://dx.doi.org/10.1038/s41467-022-31270-y

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9206660/
https://www.ncbi.nlm.nih.gov/pubmed/35717416
http://dx.doi.org/10.1038/s41467-022-31270-y

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