In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly

The synthesis of single-stranded riboprobes or double-stranded RNAs for in situ hybridization and gene knockdowns often use vectors that require time-consuming plasmid restriction digests and inefficient gel purifications. Here, we present a faster protocol for the simultaneous plasmid restriction d...

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Detalles Bibliográficos
Autores principales: Wolff, Andrew, Wagner, Cynthia, Wolf, Julia, Lobo, Daniel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207569/
https://www.ncbi.nlm.nih.gov/pubmed/35733605
http://dx.doi.org/10.1016/j.xpro.2022.101458

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207569/
https://www.ncbi.nlm.nih.gov/pubmed/35733605
http://dx.doi.org/10.1016/j.xpro.2022.101458

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