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In situ probe and inhibitory RNA synthesis using streamlined gene cloning with Gibson assembly
The synthesis of single-stranded riboprobes or double-stranded RNAs for in situ hybridization and gene knockdowns often use vectors that require time-consuming plasmid restriction digests and inefficient gel purifications. Here, we present a faster protocol for the simultaneous plasmid restriction d...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207569/ https://www.ncbi.nlm.nih.gov/pubmed/35733605 http://dx.doi.org/10.1016/j.xpro.2022.101458 |