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High-throughput single-cell antibody secretion quantification and enrichment using droplet microfluidics-based FRET assay

High-throughput screening and enrichment of antibody-producing cells have many important applications. Herein, we present a droplet microfluidic approach for high-throughput screening and sorting of antibody-secreting cells using a Förster resonance electron transfer (FRET)-based assay. The FRET sig...

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Detalles Bibliográficos
Autores principales: Rutkauskaite, Justina, Berger, Simon, Stavrakis, Stavros, Dressler, Oliver, Heyman, John, Casadevall i Solvas, Xavier, deMello, Andrew, Mazutis, Linas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9207670/
https://www.ncbi.nlm.nih.gov/pubmed/35733793
http://dx.doi.org/10.1016/j.isci.2022.104515