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Rapid 40 kb Genome Construction from 52 Parts through Data-optimized Assembly Design

[Image: see text] Large DNA constructs (>10 kb) are invaluable tools for genetic engineering and the development of therapeutics. However, the manufacture of these constructs is laborious, often involving multiple hierarchical rounds of preparation. To address this problem, we sought to test whet...

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Detalles Bibliográficos
Autores principales: Pryor, John M., Potapov, Vladimir, Bilotti, Katharina, Pokhrel, Nilisha, Lohman, Gregory J. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208013/
https://www.ncbi.nlm.nih.gov/pubmed/35613368
http://dx.doi.org/10.1021/acssynbio.1c00525