Efficient Single-Nucleotide Microbial Genome Editing Achieved Using CRISPR/Cpf1 with Maximally 3′-End-Truncated crRNAs

[Image: see text] Mismatch tolerance, a cause of the off-target effect, impedes accurate genome editing with the CRISPR/Cas system. Herein, we observed that oligonucleotide-directed single-base substitutions could be rarely introduced in the microbial genome using CRISPR/Cpf1-mediated negative selec...

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Detalles Bibliográficos
Autores principales: Lee, Ho Joung, Kim, Hyun Ju, Park, Young-Jun, Lee, Sang Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208014/
https://www.ncbi.nlm.nih.gov/pubmed/35584409
http://dx.doi.org/10.1021/acssynbio.2c00054

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208014/
https://www.ncbi.nlm.nih.gov/pubmed/35584409
http://dx.doi.org/10.1021/acssynbio.2c00054

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