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Characterization of ColE1 Production for Robust tolC Plate Dual-Selection in E. coli
[Image: see text] Bacterial selection is an indispensable tool for E. coli genetic engineering. Marker genes allow for mutant isolation even at low editing efficiencies. TolC is an especially useful E. coli marker: its presence can be selected for with sodium dodecyl sulfate, while its absence can b...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208019/ https://www.ncbi.nlm.nih.gov/pubmed/35666547 http://dx.doi.org/10.1021/acssynbio.2c00061 |