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Characterization of ColE1 Production for Robust tolC Plate Dual-Selection in E. coli

[Image: see text] Bacterial selection is an indispensable tool for E. coli genetic engineering. Marker genes allow for mutant isolation even at low editing efficiencies. TolC is an especially useful E. coli marker: its presence can be selected for with sodium dodecyl sulfate, while its absence can b...

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Detalles Bibliográficos
Autores principales: Baas-Thomas, Maximilien S., Oehm, Sebastian B., Ostrov, Nili, Church, George M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9208019/
https://www.ncbi.nlm.nih.gov/pubmed/35666547
http://dx.doi.org/10.1021/acssynbio.2c00061