Improved and Flexible HDR Editing by Targeting Introns in iPSCs

Highly efficient gene knockout (KO) editing of CRISPR–Cas9 has been achieved in iPSCs, whereas homology-directed repair (HDR)-mediated precise gene knock-in (KI) and high-level expression are still bottlenecks for the clinical applications of iPSCs. Here, we developed a novel editing strategy that t...

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Detalles Bibliográficos
Autores principales: Fu, Juan, Fu, Ya-Wen, Zhao, Juan-Juan, Yang, Zhi-Xue, Li, Si-Ang, Li, Guo-Hua, Quan, Zi-Jun, Zhang, Feng, Zhang, Jian-Ping, Zhang, Xiao-Bing, Sun, Chang-Kai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209395/
https://www.ncbi.nlm.nih.gov/pubmed/35089463
http://dx.doi.org/10.1007/s12015-022-10331-1

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9209395/
https://www.ncbi.nlm.nih.gov/pubmed/35089463
http://dx.doi.org/10.1007/s12015-022-10331-1

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