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Multiplexed site-specific genome engineering in Mycolicibacterium neoaurum by Att/Int system
Genomic integration of genes and pathway-sized DNA cassettes is often an indispensable way to construct robust and productive microbial cell factories. For some uncommon microbial hosts, such as Mycolicibacterium and Mycobacterium species, however, it is a challenge. Here, we present a multiplexed i...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
KeAi Publishing
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213222/ https://www.ncbi.nlm.nih.gov/pubmed/35782483 http://dx.doi.org/10.1016/j.synbio.2022.05.006 |