Efficient transgenesis and homology-directed gene targeting in monolayers of primary human small intestinal and colonic epithelial stem cells

Two-dimensional (2D) cultures of intestinal and colonic epithelium can be generated using human intestinal stem cells (hISCs) derived from primary tissue sources. These 2D cultures are emerging as attractive and versatile alternatives to three-dimensional organoid cultures; however, transgenesis and...

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Detalles Bibliográficos
Autores principales: Breau, Keith A., Ok, Meryem T., Gomez-Martinez, Ismael, Burclaff, Joseph, Kohn, Nathan P., Magness, Scott T.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Resource
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213823/
https://www.ncbi.nlm.nih.gov/pubmed/35523179
http://dx.doi.org/10.1016/j.stemcr.2022.04.005

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213823/
https://www.ncbi.nlm.nih.gov/pubmed/35523179
http://dx.doi.org/10.1016/j.stemcr.2022.04.005

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