Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell surface ELISA

EGFR cell surface density, stability, internalization, and recycling can be measured by cell surface ELISA (cs-ELISA). Performing this experiment on ice impedes receptor internalization; thus the physiological cell surface receptor levels can be measured by cs-ELISA. Cell surface EGFR levels are det...

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Detalles Bibliográficos
Autores principales: Kazan, Jalal M., Pollato-Blanco, Ariadna, Lukacs, Gergely L., Pause, Arnim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213824/
https://www.ncbi.nlm.nih.gov/pubmed/35755125
http://dx.doi.org/10.1016/j.xpro.2022.101475

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213824/
https://www.ncbi.nlm.nih.gov/pubmed/35755125
http://dx.doi.org/10.1016/j.xpro.2022.101475

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