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Measuring EGFR plasma membrane density, stability, internalization, and recycling in alive adherent cells by cell surface ELISA
EGFR cell surface density, stability, internalization, and recycling can be measured by cell surface ELISA (cs-ELISA). Performing this experiment on ice impedes receptor internalization; thus the physiological cell surface receptor levels can be measured by cs-ELISA. Cell surface EGFR levels are det...
| Autores principales: | , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213824/ https://www.ncbi.nlm.nih.gov/pubmed/35755125 http://dx.doi.org/10.1016/j.xpro.2022.101475 |