Creation of Cross-Linked Crystals With Intermolecular Disulfide Bonds Connecting Symmetry-Related Molecules Allows Retention of Tertiary Structure in Different Solvent Conditions

Protein crystals are generally fragile and sensitive to subtle changes such as pH, ionic strength, and/or temperature in their crystallization mother liquor. Here, using T4 phage lysozyme as a model protein, the three-dimensional rigidification of protein crystals was conducted by introducing disulf...

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Detalles Bibliográficos
Autores principales: Hiromoto, Takeshi, Ikura, Teikichi, Honjo, Eijiro, Blaber, Michael, Kuroki, Ryota, Tamada, Taro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Molecular Biosciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213883/
https://www.ncbi.nlm.nih.gov/pubmed/35755825
http://dx.doi.org/10.3389/fmolb.2022.908394

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9213883/
https://www.ncbi.nlm.nih.gov/pubmed/35755825
http://dx.doi.org/10.3389/fmolb.2022.908394

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