Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages

Plasma-treated media (PTM) serve as an adjuvant therapy to postoperatively remove residual cancerous lesions. We speculated that PTM could selectively kill cells infected with Mycobacterium tuberculosis (Mtb) and remove postoperative residual tuberculous lesions. We therefore investigated the effect...

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Autores principales: Lee, Chae Bok, Lee, Kang In, Kim, Young Jae, Jang, In Taek, Gurmessa, Sintayehu Kebede, Choi, Eun Ha, Kaushik, Nagendra Kumar, Kim, Hwa-Jung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219627/
https://www.ncbi.nlm.nih.gov/pubmed/35740265
http://dx.doi.org/10.3390/biomedicines10061243
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author Lee, Chae Bok
Lee, Kang In
Kim, Young Jae
Jang, In Taek
Gurmessa, Sintayehu Kebede
Choi, Eun Ha
Kaushik, Nagendra Kumar
Kim, Hwa-Jung
author_facet Lee, Chae Bok
Lee, Kang In
Kim, Young Jae
Jang, In Taek
Gurmessa, Sintayehu Kebede
Choi, Eun Ha
Kaushik, Nagendra Kumar
Kim, Hwa-Jung
author_sort Lee, Chae Bok
collection PubMed
description Plasma-treated media (PTM) serve as an adjuvant therapy to postoperatively remove residual cancerous lesions. We speculated that PTM could selectively kill cells infected with Mycobacterium tuberculosis (Mtb) and remove postoperative residual tuberculous lesions. We therefore investigated the effects of a medium exposed to a non-thermal plasma jet on the suppression of intracellular Mtb replication, cell death, signaling, and selectivity. We propose that PTM elevates the levels of the detoxifying enzymes, glutathione peroxidase, catalase, and ataxia-telangiectasia mutated serine/threonine kinase and increases intracellular reactive oxygen species production in Mtb-infected cells. The bacterial load was significantly decreased in spleen and lung tissues and single-cell suspensions from mice intraperitoneally injected with PTM compared with saline and untreated medium. Therefore, PTM has the potential as a novel treatment that can eliminate residual Mtb-infected cells after infected tissues are surgically resected.
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spelling pubmed-92196272022-06-24 Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages Lee, Chae Bok Lee, Kang In Kim, Young Jae Jang, In Taek Gurmessa, Sintayehu Kebede Choi, Eun Ha Kaushik, Nagendra Kumar Kim, Hwa-Jung Biomedicines Article Plasma-treated media (PTM) serve as an adjuvant therapy to postoperatively remove residual cancerous lesions. We speculated that PTM could selectively kill cells infected with Mycobacterium tuberculosis (Mtb) and remove postoperative residual tuberculous lesions. We therefore investigated the effects of a medium exposed to a non-thermal plasma jet on the suppression of intracellular Mtb replication, cell death, signaling, and selectivity. We propose that PTM elevates the levels of the detoxifying enzymes, glutathione peroxidase, catalase, and ataxia-telangiectasia mutated serine/threonine kinase and increases intracellular reactive oxygen species production in Mtb-infected cells. The bacterial load was significantly decreased in spleen and lung tissues and single-cell suspensions from mice intraperitoneally injected with PTM compared with saline and untreated medium. Therefore, PTM has the potential as a novel treatment that can eliminate residual Mtb-infected cells after infected tissues are surgically resected. MDPI 2022-05-26 /pmc/articles/PMC9219627/ /pubmed/35740265 http://dx.doi.org/10.3390/biomedicines10061243 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lee, Chae Bok
Lee, Kang In
Kim, Young Jae
Jang, In Taek
Gurmessa, Sintayehu Kebede
Choi, Eun Ha
Kaushik, Nagendra Kumar
Kim, Hwa-Jung
Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title_full Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title_fullStr Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title_full_unstemmed Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title_short Non-Thermal Plasma Jet-Treated Medium Induces Selective Cytotoxicity against Mycobacterium tuberculosis-Infected Macrophages
title_sort non-thermal plasma jet-treated medium induces selective cytotoxicity against mycobacterium tuberculosis-infected macrophages
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9219627/
https://www.ncbi.nlm.nih.gov/pubmed/35740265
http://dx.doi.org/10.3390/biomedicines10061243
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