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An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR

Traditional qPCR instrument is combined with CMOS and a personal computer, and a photoelectric feedback automatic fluorescence detection system is designed to realize quantitative real-time PCR. The key to reaction efficiency lies in how to ensure that the temperature of the detection reagent comple...

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Detalles Bibliográficos
Autores principales: Wang, Kangning, Jiang, Yangyang, Guo, Yu, Geng, Mingkun, Wu, Wenming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220086/
https://www.ncbi.nlm.nih.gov/pubmed/35735480
http://dx.doi.org/10.3390/bioengineering9060237