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An Optimized Thermal Feedback Methodology for Accurate Temperature Control and High Amplification Efficiency during Fluorescent qPCR
Traditional qPCR instrument is combined with CMOS and a personal computer, and a photoelectric feedback automatic fluorescence detection system is designed to realize quantitative real-time PCR. The key to reaction efficiency lies in how to ensure that the temperature of the detection reagent comple...
Autores principales: | Wang, Kangning, Jiang, Yangyang, Guo, Yu, Geng, Mingkun, Wu, Wenming |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9220086/ https://www.ncbi.nlm.nih.gov/pubmed/35735480 http://dx.doi.org/10.3390/bioengineering9060237 |
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